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101.
The first gene assignment to a horse chromosome is reported for equine leucocyte antigen (ELA), the major histocompatibility complex of the horse. A cloned DNA sequence derived from a class I gene of the porcine major histocompatibility complex was used as a probe for an in situ hybridization experiment. We present the regional localization of ELA, using this sequence, to equine chromosome 20g14-q22. 相似文献
102.
Studies of esterase 6 in Drosophila melanogaster. XVIII. Biochemical differences between the slow and fast allozymes 总被引:1,自引:0,他引:1
Most natural populations of Drosophila melanogaster are polymorphic for two
major electrophoretic variants at the esterase-6 locus. The frequency of
the EST 6F allozyme is greatest in populations in warmer latitudes, whereas
the EST 6S allozyme is predominant in colder latitudes. Latitudinal clines
in electromorph frequencies are found on three continents. Purified
preparations of the allozymes have been characterized for their pH optimum,
substrate specificity, organophosphate inhibition, alcohol activation,
thermal stability, and kinetic parameters. These and previous analyses of
the EST 6 allozymes reveal that the two variants have differences in their
physical and kinetic properties that may provide a basis for the selective
maintenance of the polymorphisms and an explanation of the clinal variation
observed in natural populations.
相似文献
103.
Khalid Eljaaly Asalah Helal Tamather Almandeel Rawan Algarni Samah Alshehri 《Saudi Journal of Biological Sciences》2021,28(12):6929-6932
IntroductionOral fluoroquinolones and tetracyclines are known to interact with divalent or trivalent cation-containing compounds (DTCCs) via chelation. The objective of this study is to describe the prevalence of these drug-drug interactions (DDIs) in an inpatient setting.MethodsA cross-sectional study of prospectively collected data were conducted at an academic tertiary care hospital. We included hospitalized adults who were receiving oral fluoroquinolones or tetracyclines with DTCCs in 2019. Our hospital uses electronic health records for medication ordering and handwritten medication administration records (MARs). The primary study outcome was the percentage of simultaneous administration of fluoroquinolones or tetracyclines with DTCCs, and the secondary outcome was the percentage of inappropriate separation time.ResultsAmong patients who received oral fluoroquinolones or tetracyclines, 47 patients (26.6%) were co-administered DTCCs and included in this study. Ciprofloxacin (n = 29; 61.7%) was the most commonly interacting antibiotic, followed by moxifloxacin (n = 12; 25.5%) and doxycycline (n = 6; 12.8%). The interacting DTCCs included iron-containing products and calcium-containing products, and half of the patients (n = 24; 51%) received DTCCs once daily. Most patients (n = 35; 74.5%) were found to receive oral fluoroquinolones or tetracyclines at the same time as DTCCs, while one (2.1%) received inappropriately separated DTCCs.ConclusionsDespite being a very known contraindicated DDI, the prevalence of simultaneous co-administration of oral fluoroquinolones or tetracyclines with polyvalent cations was extremely high in a hospital with handwritten MARs. Antimicrobial stewardship programs should target this DDI, and future studies should evaluate the impact of different practical solutions to this problem in different clinical settings. 相似文献
104.
Racha Helal Matthias F. Melzig 《In vitro cellular & developmental biology. Animal》2010,46(6):492-496
The main purpose of this study was to investigate lysozyme synthesis and secretion in three human monocyte cell lines: U-937,
HL-60, and THP-1, using sensitive fluorescence-based assay of lysozyme activity. PMA and hIFN-γ were evaluated for inducing
lysozyme activity. Using well-defined cell lines from the cell culture collection, no lysozyme activity could be detected
in the cultured U-937 cells either with or without addition of the inducing factors. These data suggested, contrary to previous
reports, that U-937 cell line cannot synthesize or secrete active lysozyme. THP-1 and HL-60 cells were proved to produce enzymatically
active lysozyme in increasing amounts with the time course. PMA and hIFN-γ had no significant inducing effect on the production
or the release of active lysozyme in THP-1 and HL-60 cells. We showed inhibiting effect of PMA and hIFN-γ on the lysozyme
activity, particularly in HL-60 cell line. 相似文献
105.
106.
Biswas MH Hasegawa HH Rahman MA Huang P Mon NN Ruhul Amin AR Senga T Kannagi R Hamaguchi M 《Biochemical and biophysical research communications》2006,348(3):1145-1149
To search for the signaling critical for the production of tissue inhibitor of metalloproteinase-2 (TIMP-2), we investigated the role of SHP-2 in TIMP-2 production with Concanavalin A (Con A)-treated cells. In wild-type fibroblasts, Con A-treatment dramatically activated TIMP-2 production. In contrast, production of TIMP-2 in response to Con A-treatment was severely impaired in cells expressing mutant SHP-2 whose 65 amino acids in the SH2-N domain were deleted. Con A-treatment activated dual signaling pathways, Erk and p38, in a SHP-2-dependent manner. Pretreatment of wild-type cells with U0126, a potent inhibitor of MEK1, significantly inhibited the production of TIMP-2, whereas SB203580, a specific inhibitor for p38, could not. Finally, expression of exogenous wild-type SHP-2 in SHP-2 mutant cells clearly rescued Erk activation and TIMP-2 production in response to Con A-treatment. Taken together, our results strongly suggest that SHP-2 plays a critical role as a positive modulator for the production of TIMP-2 via MEK1-Erk signaling in fibroblasts. 相似文献
107.
Boussaada O Ammar S Saidana D Chriaa J Chraif I Daami M Helal AN Mighri Z 《Microbiological research》2008,163(1):87-95
The chemical composition of the volatile fractions obtained by steam distillation from the capitula (C) and the aerial parts of Rhaponticum acaule DC were analysed by GC-MS. From the 57 identified constituents, representing 95.5% and 96.3% of the two oils, respectively, methyl eugenol, epi-13 manool, beta-ionone, beta-bisabolol, 1-octadecanol, phytol and farnesyl acetate were found to be the main components. Furthermore, the oils were tested against six Gram-positive and Gram-negative bacteria and four phytopathogenic fungi. It was found that oils from both parts of R. acaule, and especially that of C, exhibited interesting antibacterial activity, but no antifungal activity was observed. 相似文献
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